Electron acceptance at photosystem II in an oxygen-evolving photosystem II preparation: clear discrimination of two sites of electron acceptance for quinones and quinonediimines associated with a photosystem II preparation.

Oxygen-producing electron transport reactions of a photosystem II preparation are described. The preparation has six major peptides with apparent molecular weights of 36,000, 31,000, 28,000, 27,000, 25,000, and 21,000. Sucrose density gradient centrifugation indicates that the preparation is more homogeneous and more dense than control thylakoid membranes. The preparation photoreduces a number of known photosystem II oxidants including the Class I acceptor, ferricyanide; the Class II acceptor, 2,6-dichloroindophenol; and the Class III acceptor, 2,6-dichlorobenzoquinone. However, quinonediimines such as phenylenediimine are not reduced, suggesting that these substances are reduced at sites in the thylakoid membrane which are not found in the photosystem II preparation. All the oxygen-producing reactions are sensitive to inhibition by 3-(3,4-dichlorophenyl)-1,1-dimethylurea.