一种精确且灵敏的测量脊髓血流量的方法。

A precise and sensitive method for measurement of spinal cord blood flow.

作者信息

Zivin J A, Waud D R

出版信息

Brain Res. 1983 Jan 10;258(2):197-200. doi: 10.1016/0006-8993(83)91142-3.

Abstract

Spinal cord blood flow can be accurately and rapidly measured in rabbits by intravenous injection of [14C]iodoantipyrine, collection of sequential arterial samples, and abrupt termination of blood flow to the spinal cord by severing the aorta with a previously implanted ligature. The radioactive tracer concentrations in arterial and spinal cord samples can be rapidly measured in a scintillation counter. The spinal cord blood flow can then be calculated from these blood and tissue tracer concentrations. This method is suitable for use in anesthetized or unanesthetized small animals.

摘要

通过静脉注射[14C]碘安替比林、收集连续的动脉样本以及用先前植入的结扎线切断主动脉使脊髓血流突然中断，可准确快速地测量兔脊髓血流量。动脉和脊髓样本中的放射性示踪剂浓度可在闪烁计数器中快速测量。然后可根据这些血液和组织示踪剂浓度计算脊髓血流量。该方法适用于麻醉或未麻醉的小动物。