Kaung H C
Gen Comp Endocrinol. 1983 Jan;49(1):50-6. doi: 10.1016/0016-6480(83)90006-0.
The morphology and population size of immunohistochemically stained insulin-containing pancreatic beta cells are studied in Rana pipiens during larval development. These beta cells appear as single cells or form small clusters at early stages and gradually develop into large clusters arranged in cords surrounding the blood sinusoids in midlarval stages. The total quantity of these cells increases from stage II through midlarval stages, reaching maximum at stages XVIII and XIX. A significant decrease of total beta cell quantity is observed between stages XIX and XX. This decrease occurs slightly later than the onset of degeneration of exocrine tissue. Morphological indication of beta cell breakdown is also observed during this period. Although the population size of beta cells stays relatively constant from stages XXI to XXV, a further maturational change is observed in beta cell morphology between stages XXIV and XXV. During these stages, the polarization of stained immunoreactivity toward sinusoids is more obvious and there is a general decrease of staining intensity in the cytoplasm.
在牛蛙幼体发育过程中，对免疫组织化学染色的含胰岛素胰腺β细胞的形态和数量进行了研究。这些β细胞在早期表现为单个细胞或形成小簇，在幼体中期逐渐发展成围绕血窦排列成索状的大簇。这些细胞的总数从II期到幼体中期增加，在XVIII和XIX期达到最大值。在XIX期和XX期之间观察到β细胞总数显著减少。这种减少比外分泌组织退化的开始稍晚发生。在此期间也观察到β细胞解体的形态学迹象。尽管从XXI期到XXV期β细胞数量相对保持恒定，但在XXIV期和XXV期之间观察到β细胞形态有进一步的成熟变化。在这些阶段，染色免疫反应性向血窦的极化更明显，细胞质中的染色强度普遍降低。
