在无蛋白培养基中进行单克隆抗体的常规大规模生产。 - Suppr

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Routine large-scale production of monoclonal antibodies in a protein-free culture medium.

作者信息

Cleveland W L, Wood I, Erlanger B F

出版信息

J Immunol Methods. 1983 Jan 28;56(2):221-34. doi: 10.1016/0022-1759(83)90414-3.

DOI:10.1016/0022-1759(83)90414-3

PMID:6338115

Abstract

摘要

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Routine large-scale production of monoclonal antibodies in a protein-free culture medium.在无蛋白培养基中进行单克隆抗体的常规大规模生产。

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引用本文的文献

Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors.无血清生长培养基，用于搅拌式生物反应器中培养多种哺乳动物细胞。

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Large scale transient expression with COS cells.用 COS 细胞进行大规模瞬时表达。

Cytotechnology. 1995 Jan;18(3):183-92. doi: 10.1007/BF00767766.

Evaluation of a simple protein free medium that supports high levels of monoclonal antibody production.评估一种简单的无蛋白培养基，该培养基能够支持高水平的单克隆抗体生产。

Cytotechnology. 1996 Jun;21(2):95-109. doi: 10.1007/BF02215660.

The culture of rat myeloma and rat hybridoma cells in a protein-free medium.无血清培养基中大鼠骨髓瘤细胞和大鼠杂交瘤细胞的培养。

Cytotechnology. 1995 Oct;17(3):193-202. doi: 10.1007/BF00749657.

Multiclass detection of cells in multicontrast composite images.多对比度复合图像中细胞的多类别检测。

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Linoleic acid improves the robustness of cells in agitated cultures.亚油酸可提高搅拌培养细胞的稳定性。

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Unsaturated fatty acids enhance cell yields and perturb the energy metabolism of an antibody-secreting hybridoma.不饱和脂肪酸可提高细胞产量，并扰乱分泌抗体的杂交瘤的能量代谢。

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Serum-free medium for long-term growth, freezing, cloning and fusion of myelomas and hybridomas.用于骨髓瘤和杂交瘤长期生长、冷冻、克隆及融合的无血清培养基。

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Substitution of transferrin by FeCl3 in the development of a low foetal calf serum concentration medium for KB-26.5 hybridoma cell line.在开发用于KB - 26.5杂交瘤细胞系的低胎牛血清浓度培养基过程中用三氯化铁替代转铁蛋白。

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Growth of cells in a new defined protein-free medium.细胞在一种新的限定无蛋白培养基中的生长。

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