Löfdahl S, Guss B, Uhlén M, Philipson L, Lindberg M
Proc Natl Acad Sci U S A. 1983 Feb;80(3):697-701. doi: 10.1073/pnas.80.3.697.
The gene for protein A from Staphylococcus aureus was cloned into pBR322 in Escherichia coli. An immunoassay was used to detect production of the protein. Protein A produced in E. coli was found in the periplasmic space and was purified and concentrated by IgG-Sepharose affinity chromatography. DNA sequence assay of the gene revealed a region with the general features of a prokaryotic signal peptide and a fifth structural region homologous to the four repetitive regions found earlier by amino acid sequence determination of the mature protein. Upstream from the structural gene there is a possible promoter region and a ribosomal binding sequence typical of gram-positive bacteria. The initiation codon is TTG.
将金黄色葡萄球菌的蛋白A基因克隆到大肠杆菌的pBR322中。采用免疫测定法检测该蛋白的产生。在大肠杆菌中产生的蛋白A存在于周质空间，并通过IgG-琼脂糖亲和层析进行纯化和浓缩。该基因的DNA序列分析显示，有一个区域具有原核信号肽的一般特征，以及与先前通过成熟蛋白氨基酸序列测定发现的四个重复区域同源的第五个结构区域。在结构基因上游有一个可能的启动子区域和革兰氏阳性细菌典型的核糖体结合序列。起始密码子是TTG。
