Ohkawa T
Biochim Biophys Acta. 1983 Apr 20;756(3):335-40. doi: 10.1016/0304-4165(83)90342-2.
From Escherichia coli K12 W2252-11U-cells, the Ter-15 mutant, the Ter-15 (F'-lac) and the Ter-15 (F+) cells, lipopolysaccharides were isolated and the primary structure of its core oligosaccharides was elucidated. When the F'-lac episome is transferred to the Ter-15 mutant by conjugation, the structure of the glucose III(1 leads to 3)glucose II(1 leads to 3)glucose I residue and the galactose I(1 leads to 2)-linked to the glucose I residue in the core oligosaccharide from the Ter-15 mutant changes into the structure of the glucose IV(1 leads to 6)glucose III(1 leads to 2)glucose II(1 leads to 3)glucose I residue and the galactose I (1 leads to 6)-linked to the glucose I residue in the core oligosaccharide from the Ter-15 (F'-lac) cells, but the core oligosaccharide in the Ter-15 (F+) cells is the same structure with that of the core oligosaccharide from the Ter-15 mutant when F+ episome is transferred to the Ter-15 mutant. Also, the core oligosaccharide from the Ter-15 (F'-lac) cells shows the same structure with that of the core oligosaccharide from E. coli K12 W2252-11U- cells (the parent cells). As the result, the ability to produce the structure of the core oligosaccharide in E. coli K12 W2252-11U- cells is recovered in the Ter-15 (F'-lac) cells by the dominant expression of lac gene or its containing DNA segment in F'-lac episome.
从大肠杆菌K12 W2252 - 11U细胞、Ter - 15突变体、Ter - 15（F'-lac）和Ter - 15（F +）细胞中分离出脂多糖，并阐明了其核心寡糖的一级结构。当F'-lac附加体通过接合转移到Ter - 15突变体时，Ter - 15突变体核心寡糖中葡萄糖III（1→3）葡萄糖II（1→3）葡萄糖I残基以及与葡萄糖I残基相连的半乳糖I（1→2）的结构，转变为Ter - 15（F'-lac）细胞核心寡糖中葡萄糖IV（1→6）葡萄糖III（1→2）葡萄糖II（1→3）葡萄糖I残基以及与葡萄糖I残基相连的半乳糖I（1→6）的结构，但是当F +附加体转移到Ter - 15突变体时，Ter - 15（F +）细胞中的核心寡糖与Ter - 15突变体核心寡糖的结构相同。此外，Ter - 15（F'-lac）细胞的核心寡糖与大肠杆菌K12 W2252 - 11U细胞（亲代细胞）的核心寡糖结构相同。结果，通过F'-lac附加体中lac基因或其包含的DNA片段的显性表达，Ter - 15（F'-lac）细胞恢复了大肠杆菌K12 W2252 - 11U细胞中产生核心寡糖结构的能力。
