Characterization of a concanavalin A-induced amplifier T cell which augments in vitro antibody responses to DNP-Ficoll.

The addition of the T-cell mitogen concanavalin A (Con A) on Day 2 of a 4-day in vitro culture of murine spleen cells with the thymus-independent (TI) antigen DNP39-Ficoll resulted in significant enhancement of the direct antitrinitrophenyl (TNP) plaque-forming cell (PFC) response. This enhancement was mediated by a nylon wool- and antiimmunoglobulin-nonadherent amplifier T cell (TA). TA activity was not eliminated by in vitro treatment of T cells with anti-Thy 1.2 and complement (C). TA activity could be eliminated by pretreatment of mice with antilymphocyte serum (ALS) in vivo, followed by in vitro treatment of T cells with anti-Thy 1.2 + C. Thus, TA appear to bear a low surface density of Thy-1 antigen. These TA were relatively resistant to ALS used alone, to cyclophosphamide, and to low dose in vitro irradiation. TA were still present in the spleen 14 weeks after adult thymectomy (ATx). They were I-J positive and apparently belonged to the Lyt 1+2- T-cell subset.