Finesilver A G, Braley-Mullen H
Cell Immunol. 1983 Feb 1;75(2):199-213. doi: 10.1016/0008-8749(83)90320-9.
The addition of the T-cell mitogen concanavalin A (Con A) on Day 2 of a 4-day in vitro culture of murine spleen cells with the thymus-independent (TI) antigen DNP39-Ficoll resulted in significant enhancement of the direct antitrinitrophenyl (TNP) plaque-forming cell (PFC) response. This enhancement was mediated by a nylon wool- and antiimmunoglobulin-nonadherent amplifier T cell (TA). TA activity was not eliminated by in vitro treatment of T cells with anti-Thy 1.2 and complement (C). TA activity could be eliminated by pretreatment of mice with antilymphocyte serum (ALS) in vivo, followed by in vitro treatment of T cells with anti-Thy 1.2 + C. Thus, TA appear to bear a low surface density of Thy-1 antigen. These TA were relatively resistant to ALS used alone, to cyclophosphamide, and to low dose in vitro irradiation. TA were still present in the spleen 14 weeks after adult thymectomy (ATx). They were I-J positive and apparently belonged to the Lyt 1+2- T-cell subset.
在将鼠脾细胞与非胸腺依赖性（TI）抗原DNP39-菲可进行为期4天的体外培养的第2天添加T细胞丝裂原伴刀豆球蛋白A（Con A），可显著增强直接抗三硝基苯（TNP）空斑形成细胞（PFC）反应。这种增强作用由一种尼龙毛和抗免疫球蛋白非黏附性放大T细胞（TA）介导。用抗Thy 1.2和补体（C）对T细胞进行体外处理并不能消除TA活性。通过在体内用抗淋巴细胞血清（ALS）预处理小鼠，然后在体外用抗Thy 1.2 + C处理T细胞，可以消除TA活性。因此，TA似乎具有低表面密度的Thy-1抗原。这些TA对单独使用的ALS、环磷酰胺和低剂量体外照射相对抗性。成年胸腺切除（ATx）14周后，TA仍存在于脾脏中。它们I-J阳性，显然属于Lyt 1+2-T细胞亚群。
