Stockem W, Naib-Majani W, Wohlfarth-Bottermann K E, Osborn M, Weber K
Eur J Cell Biol. 1983 Jan;29(2):171-8.
The spatial distribution of cytoplasmic actin and myosin in 1. normal locomoting, 2. immobilized, and 3. pinocytosing Amoeba proteus was demonstrated by indirect immunofluorescence microscopy. In orthotactic and polytactic cells fixed during normal locomotion actin is mainly located in a cortical layer delineating the granuloplasm from the peripheral hyaloplasm. In cell areas lacking a hyaloplasmic sheet the actin layer immediately borders the plasma membrane. The amount of actin within the continuous layer seems to increase from the advancing front to the middle cell region and to decrease again toward the uroid. The distribution of myosin is largely congruent to the display of actin, with the exception that the myosin-based fluorescence of the cortical layer gradually increases from the front to the uroid. A considerable amount of actin and myosin is also distributed around the nucleus and the contractile vacuole. In immobilized cells contracted by the external application of 10(-4)M procaine hydrochloride the cortical layer distinctly increases in thickness. In contrast to normal locomoting cells actin and myosin show a uniform distribution within the cell cortex along the entire surface. In pinocytosing cells, up to three cortical layers conspicuously rich in actin are produced during the process of channel formation. One of these layers is located in close proximity to the plasma membrane of the pinocytotic channels and the vacuoles. The immunocytochemical results are discussed with respect to earlier observations on the distribution of actin and myosin in Amoeba proteus as obtained by other methods.
通过间接免疫荧光显微镜观察，证实了变形虫在1. 正常移动、2. 固定以及3. 胞饮过程中细胞质肌动蛋白和肌球蛋白的空间分布。在正常移动过程中固定的正向移动和多向移动细胞中，肌动蛋白主要位于皮质层，该皮质层将颗粒质与外周透明质质分隔开来。在缺乏透明质质片层的细胞区域，肌动蛋白层紧邻质膜。连续层内的肌动蛋白量似乎从前进前沿向细胞中部区域增加，然后向尾状部再次减少。肌球蛋白的分布与肌动蛋白的分布基本一致，只是皮质层基于肌球蛋白的荧光从前向后逐渐增加。相当数量的肌动蛋白和肌球蛋白也分布在细胞核和收缩泡周围。在通过外部施加10(-4)M盐酸普鲁卡因而收缩的固定细胞中，皮质层厚度明显增加。与正常移动细胞相比，肌动蛋白和肌球蛋白在整个细胞表面的细胞皮质内呈现均匀分布。在胞饮细胞中，在通道形成过程中会产生多达三个明显富含肌动蛋白的皮质层。其中一层紧邻胞饮通道和液泡的质膜。结合其他方法对变形虫中肌动蛋白和肌球蛋白分布的早期观察结果，对免疫细胞化学结果进行了讨论。
