Kobayashi N, Okita M, Yarita T, Hanzawa S, Okamoto T, Katsuki H
J Thorac Cardiovasc Surg. 1978 Mar;75(3):434-42.
A method for induction of carcinoma in subcutaneously implanted bronchial autografts in dogs is described. Four to 6 weeks after successful bronchial autografting, 30 to 90 mg. amounts of 3-MC or B(a)P suspended in sterile saline solution containing 1 percent CMC was injected into the lumen of the graft. The mucosal changes were followed histologically by excision of biopsy specimens and cytologically by needle aspiration biopsies. Three dogs developed squamous cell carcinomas invading deep into the submucosal area as early as 13 to 30 weeks after the first carcinogen treatment. The remaining eight experimental dogs showed various grades of squamous cell metaplasia with atypia within 30 weeks. Subsequently examined specimens revealed atypical metaplastic and proliferative changes further advanced than those seen in the initial biopsy specimens. The advantages of this method for a better understanding of the development of bronchogenic carcinomas are discussed.
本文描述了一种在犬皮下植入支气管自体移植物中诱导癌的方法。在成功进行支气管自体移植4至6周后，将30至90毫克悬浮于含1%羧甲基纤维素的无菌盐溶液中的3-甲基胆蒽或苯并(a)芘注入移植物腔内。通过切除活检标本进行组织学观察黏膜变化，并通过针吸活检进行细胞学观察。最早在首次致癌物处理后13至30周，3只犬发生了浸润至黏膜下深层区域的鳞状细胞癌。其余8只实验犬在30周内表现出不同程度的伴有异型性的鳞状上皮化生。随后检查的标本显示，非典型化生和增殖性变化比初始活检标本中所见的更为进展。讨论了该方法对于更好地理解支气管源性癌发生发展的优势。
