Higaki J, Hirose S, Ogihara T, Imai N, Kisaragi M, Murakami K, Kumahara Y
Life Sci. 1983 Apr 4;32(14):1591-8. doi: 10.1016/0024-3205(83)90865-2.
Human renal renin was isolated from a partially purified preparation, Haas' preparation step 5 material, with a remarkably high yield of 28% by a newly developed method. This method consisted of only four steps including affinity chromatography on pepstatin-aminohexyl-agarose, chromatofocusing, gel filtration and DEAE-chromatography after the initial extraction and concentration. This method enabled us to obtain pure human renin without another affinity column used by other investigators. Pure human renin had a molecular weight of 40,000 daltons as estimated by gel filtration and sodiumdodecylsulfate-polyacrylamide gel electrophoresis. The pI of purified renin was 5.6.
人肾素是从部分纯化的制剂（哈斯制备步骤5的材料）中分离出来的，采用一种新开发的方法，产率高达28%。该方法仅包括四个步骤，即在初始提取和浓缩后，依次进行胃蛋白酶抑制剂-氨基己基琼脂糖亲和层析、色谱聚焦、凝胶过滤和DEAE层析。该方法使我们无需使用其他研究者所用的另一亲和柱就能获得纯人肾素。通过凝胶过滤和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳估计，纯人肾素的分子量为40,000道尔顿。纯化肾素的pI为5.6。
