全氯乙烯的体外和体内遗传与生化研究。

Genetic and biochemical studies on perchloroethylene 'in vitro' and 'in vivo'.

作者信息

Bronzetti G, Bauer C, Corsi C, Del Carratore R, Galli A, Nieri R, Paolini M

出版信息

Mutat Res. 1983 Mar;116(3-4):323-31. doi: 10.1016/0165-1218(83)90070-8.

Abstract

Perchloroethylene (PCE) was tested in a diploid strain (D7) of the yeast Saccharomyces cerevisiae in suspension tests with and without a mammalian microsomal activation system (S9) and 'in vivo' by the intrasanguineous host-mediated assay. In addition, enzyme alteration studies were performed in mice non-pretreated or pretreated with phenobarbital + beta-naphthoflavone. PCE did not induce any genetic effect either 'in vitro' or 'in vivo'. In the suspension test, PCE was more toxic without metabolic activation and less toxic with mammalian microsomal activation. The enzymatic determinations showed an increase of the aminopyrine demethylase activity and of the level of cytochrome P-450.

摘要

在酿酒酵母的二倍体菌株（D7）中，采用悬浮试验，在有和没有哺乳动物微粒体激活系统（S9）的情况下对全氯乙烯（PCE）进行了测试，并通过血内宿主介导试验进行了“体内”测试。此外，还对未用苯巴比妥 + β-萘黄酮预处理或预处理过的小鼠进行了酶改变研究。PCE在“体外”或“体内”均未诱导任何遗传效应。在悬浮试验中，PCE在没有代谢激活时毒性更大，在有哺乳动物微粒体激活时毒性较小。酶活性测定表明氨基比林脱甲基酶活性和细胞色素P - 450水平有所增加。