Hilinski E F, Rentzepis P M
Nature. 1983 Apr 7;302(5908):481-7. doi: 10.1038/302481a0.
Technological advances in picosecond spectroscopy have permitted the mechanisms of various chemical, physical and biological processes to be elucidated and understood to a greater degree than ever before. By means of picosecond emission, absorption and Raman spectroscopy, one can probe and measure directly the transient intermediates and kinetics of primary events in complex biological processes. A description of two current types of laser systems--solid-state and synchronously pumped dye lasers--and their application to determining the primary events in the biological processes of dissociation of oxy- and carboxymyoglobin, excited-state relaxation of porphyrins and visual transduction, illustrate the power of picosecond spectroscopy.
皮秒光谱学的技术进步使得各种化学、物理和生物过程的机制比以往任何时候都能得到更大程度的阐明和理解。通过皮秒发射、吸收和拉曼光谱，人们可以直接探测和测量复杂生物过程中初级事件的瞬态中间体和动力学。对两种当前类型的激光系统——固态激光器和同步泵浦染料激光器——及其在确定氧合肌红蛋白和羧基肌红蛋白解离的生物过程、卟啉的激发态弛豫以及视觉转导中的初级事件方面的应用进行描述，说明了皮秒光谱学的强大功能。
