Verheugen C, Laufer L R, DeFazio J L, Pardridge W B, Lu J K, Judd H L
Neuroendocrinology. 1983 Feb;36(2):102-4. doi: 10.1159/000123444.
To assess the permeability of the blood-brain barrier (BBB) to exogenously administered gondatrophin-releasing hormone (GnRH), a study was performed in male-rats using the double isotope, intracarotid, single injection technique of Oldendorf. The mean (+/- SEM) brain uptake indices (BUI) of 3H-GnRH in comparison to a freely diffusible reference compound, 14C-butanol, were 1.1 +/- 0.2, 3.5 +/- 0.7 and 3.0 +/- 0.6 when the injection vehicles were Ringer's lactate, rat serum and human serum, respectively. The BUI of 3H-GnRH was similar to that of 3H-inulin, a nondiffusible compound. With different concentrations, the BUI of 3H-GnRH did not change significantly. These data indicate that the BBB of the male rat is impermeable to exogenously administered GnRH.
为评估血脑屏障（BBB）对外源性促性腺激素释放激素（GnRH）的通透性，采用Oldendorf的双同位素、颈内动脉单次注射技术在雄性大鼠中进行了一项研究。与可自由扩散的参考化合物14C-丁醇相比，当注射媒介分别为乳酸林格液、大鼠血清和人血清时，3H-GnRH的平均（±标准误）脑摄取指数（BUI）分别为1.1±0.2、3.5±0.7和3.0±0.6。3H-GnRH的BUI与非扩散性化合物3H-菊粉的BUI相似。在不同浓度下，3H-GnRH的BUI无显著变化。这些数据表明雄性大鼠的血脑屏障对外源性GnRH是不透性的。
