Adelman B, Stemerman M B, Handin R I
Arteriosclerosis. 1983 Mar-Apr;3(2):141-8. doi: 10.1161/01.atv.3.2.141.
Following aortic balloon deendothelialization, rabbits develop a neointima composed of smooth muscle cells and extracellular connective tissue. Injury of this neointima with a balloon catheter results in the accumulation of platelet aggregates and fibrin on the vessel surface. We studied platelet attachment and secretion following injury of the neointima and also the effect of prostaglandin I2 (PGI2) and heparin on these events. Platelet factor 4 was detected within the neointima by indirect immunofluorescence 30 minutes after neointimal injury. By using 51Cr-labeled platelets, it was possible to quantitate total platelet attachment following neointimal injury. When animals were sacrificed 30 minutes after reinjury, there were 4.46 X 10(6) platelets/cm2 of aortic surface in animals injured 10 days after initial balloon deendothelialization, and 3.75 X 10(6) platelets/cm2 of aortic surface in animals injured 29 days after initial injury. In these two groups, infusion of 850 ng/kg/min PGI2, along with a single infusion of 2500 units of heparin, inhibited fibrin deposition and reduced platelet attachment by 71% and 76%, respectively. Although infusion of heparin alone prevented fibrin deposition, neither heparin nor PGI2 individually reduced platelet attachment as profoundly as did their combined use.
在主动脉球囊去内皮化后，兔子会形成由平滑肌细胞和细胞外结缔组织组成的新生内膜。用球囊导管损伤该新生内膜会导致血小板聚集体和纤维蛋白在血管表面积聚。我们研究了新生内膜损伤后血小板的黏附与分泌情况，以及前列腺素I2（PGI2）和肝素对这些过程的影响。在新生内膜损伤30分钟后，通过间接免疫荧光在内膜中检测到血小板第4因子。使用51Cr标记的血小板，可以定量新生内膜损伤后总的血小板黏附情况。当动物在再次损伤30分钟后被处死时，在初次球囊去内皮化10天后受伤的动物中，主动脉表面每平方厘米有4.46×10(6)个血小板，在初次损伤29天后受伤的动物中，主动脉表面每平方厘米有3.75×10(6)个血小板。在这两组中，以850 ng/kg/min的速度输注PGI2，并单次输注2500单位肝素，可抑制纤维蛋白沉积，并分别使血小板黏附减少71%和76%。虽然单独输注肝素可防止纤维蛋白沉积，但肝素和PGI2单独使用时均不能像联合使用那样显著减少血小板黏附。
