Induction of rat liver malic enzyme messenger RNA activity by insulin and by fructose.

The effects of insulin treatment and fructose feeding on malic enzyme [EC 1.1.1.40] mRNA in liver of diabetic rats were investigated. A high fructose diet, even without insulin treatment, increased the mRNA activity, much more than a high starch diet, but to a lesser extent than insulin treatment. The increase of malic enzyme activity was approximately comparable to the change in mRNA activity, but occurred 12 hr and 30 hr after the increase of mRNA in the fructose-fed rats with and without insulin treatment, respectively. Thus, insulin and fructose appear to induce malic enzyme primarily by increasing the activity of translatable mRNA.