公羊血液和精浆中α-葡萄糖苷酶活性的比较研究。

Comparative study of alpha-glucosidase activities in blood and seminal plasma of rams.

Besancon J, Chapdelaine P, Tremblay R R, Lemay J P

Comp Biochem Physiol B. 1983;74(3):529-32. doi: 10.1016/0305-0491(83)90223-7.

Abstract

This study was undertaken to characterize the alpha-glucosidase present in blood and seminal plasma of two strains of pure-bred rams which are known as seasonal breeders. 2. pH profiles and activity levels were investigated in blood and seminal plasma using a sensitive spectrophotometric assay with para-nitrophenyl-alpha-D-glucoside as substrate. 3. According to their pH optimum, blood plasma and seminal plasma alpha-glucosidases were typically neutral and acid enzymes and significant differences were recorded in their physico-chemical properties, establishing the tissue specificity of the enzyme. 4. Notwithstanding the tissue under study, the nature of the alpha-glucosidase activity was similar in both strains of pure-bred rams.

摘要

本研究旨在表征两株纯种公羊血液和精浆中的α-葡萄糖苷酶，这两株公羊为季节性繁殖者。2. 使用对硝基苯基-α-D-葡萄糖苷作为底物的灵敏分光光度法测定血液和精浆中的pH曲线和活性水平。3. 根据其最适pH值，血浆和精浆α-葡萄糖苷酶分别为典型的中性酶和酸性酶，且其理化性质存在显著差异，证实了该酶的组织特异性。4. 无论研究的组织如何，两株纯种公羊中α-葡萄糖苷酶活性的性质相似。