Stahl S J, Stewart K R, Williams F D
J Bacteriol. 1983 May;154(2):930-7. doi: 10.1128/jb.154.2.930-937.1983.
Light microscopy, transmission electron microscopy, and scanning electron microscopy were used to visualize the extracellular slime of Proteus mirabilis swarm cells. Slime was observed with phase-contrast microscopy after fixation in hot sulfuric acid-sodium borate. Ruthenium red was used to stain slime for transmission electron microscopy. Copious quantities of extracellular slime were observed surrounding swarm cells; the slime appeared to provide a matrix through which the cells could migrate. Swarm cells were always found embedded in slime. These observations support the argument that swarming of P. mirabilis is associated with the production of large quantities of extracellular slime. Examination of nonswarming mutants of P. mirabilis revealed that a number of morphological changes, including cell elongation and increased flagellum synthesis, were required for swarm cell migration. It is still unclear whether extracellular slime production also is required for migration.
利用光学显微镜、透射电子显微镜和扫描电子显微镜观察奇异变形杆菌群游细胞的胞外黏液。在热硫酸 - 硼酸钠中固定后，用相差显微镜观察黏液。钌红用于透射电子显微镜下黏液的染色。在群游细胞周围观察到大量的胞外黏液；黏液似乎提供了一个细胞可以迁移通过的基质。群游细胞总是被发现包埋在黏液中。这些观察结果支持了奇异变形杆菌群游与大量胞外黏液产生相关的观点。对奇异变形杆菌非群游突变体的研究表明，群游细胞迁移需要一些形态学变化，包括细胞伸长和鞭毛合成增加。目前尚不清楚迁移是否也需要产生胞外黏液。
