Cheng K J, Costerton J W
Can J Microbiol. 1980 Sep;26(9):1104-13. doi: 10.1139/m80-183.
When rumen fluid is fixed for electron microscopy, without centrifugation, a large amount of material sediments spontaneously and ruthenium red staining shows this material to be partially composed of bacterial microcolonies in which morphologically similar sister cells are enclosed in an extensive fibrous exopolysaccharide glycocalyx. The exopolysaccharide matrix condenses, to variable degrees, during the dehydration steps of specimen preparation for electron microscopy but some fibers are stabilized by their attachment to solid surfaces at multiple points and the extent of this matrix can be deduced from morphological data. Even after condensation, the glycocalyces of rumen bacteria occupy more space than the cells themselves and they completely surround most cells with a fibrous matrix, whose chemical composition dictates an ion-exchange function, that must be assumed to be protective against some bacteriophage and antibacterial agents.
当瘤胃液未经离心直接用于电子显微镜固定时，大量物质会自发沉淀，钌红染色显示该物质部分由细菌微菌落组成，形态相似的姊妹细胞被包裹在广泛的纤维状胞外多糖糖萼中。在电子显微镜标本制备的脱水步骤中，胞外多糖基质会不同程度地浓缩，但一些纤维通过在多个点附着于固体表面而得以稳定，并且可以从形态学数据推断出该基质的范围。即使在浓缩后，瘤胃细菌的糖萼所占空间仍比细胞本身更大，它们用纤维状基质完全包围了大多数细胞，其化学成分决定了离子交换功能，必须假定该功能可抵御某些噬菌体和抗菌剂。
