Pavlov Iu I, Khromov-Borisov N N
Genetika. 1983 Mar;19(3):362-74.
The effect of recombinogenic and mutagenic activation of promutagens, cyclophosphamide and 2-aminofluorene was studied in S9 mouse liver preparations. Cyclophosphamide was activated to induce reversions of an ochre mutation and heteroallelic reversion in yeast tester strains r2089-15V-P4 and P3288, respectively. Metabolic activation of this chemical was greatly enhanced by pretreatment of mice with AROGLOR--1254. 2-aminofluorene was a potent recombinogen after metabolic activation, but proved a poor inducer of reversions of the ochre mutation. For the stationary yeast cells, activated 2-aminofluorene was shown to be not recombinogenic, while for logarithmic cells grown in galactose medium it was moderately recombinogenic, being highly active in this respect for logarithmic cells grown in glucose medium. We recommend to use these compounds as positive controls for exogenous activation in the yeast/microsome test.
在S9小鼠肝脏制剂中研究了前诱变剂环磷酰胺和2-氨基芴的重组和诱变激活作用。环磷酰胺被激活后，分别在酵母测试菌株r2089-15V-P4和P3288中诱导赭石突变回复和杂合等位基因回复。用AROGLOR-1254预处理小鼠可大大增强这种化学物质的代谢激活作用。2-氨基芴在代谢激活后是一种有效的重组剂，但事实证明它对赭石突变回复的诱导作用较差。对于静止的酵母细胞，活化的2-氨基芴不具有重组性，而对于在半乳糖培养基中生长的对数期细胞，它具有中等程度的重组性，在这方面对在葡萄糖培养基中生长的对数期细胞具有高活性。我们建议将这些化合物用作酵母/微粒体试验中外源激活的阳性对照。
