甲基苯丙胺的酶免疫测定法。

Enzyme immunoassay for methamphetamine.

作者信息

Aoki K, Kuroiwa Y

出版信息

J Pharmacobiodyn. 1983 Jan;6(1):33-8. doi: 10.1248/bpb1978.6.33.

DOI:10.1248/bpb1978.6.33

Abstract

A competitive enzyme immunoassay for methamphetamine with alkaline phosphatase labeled methamphetamine, Sepharose-antibody and p-nitrophenylphosphate as substrate was developed. The anti-methamphetamine antisera produced in rabbits by immunization with N-(4-aminobutyl) methamphetamine-BSA conjugate were specific for methamphetamine and showed low cross-reactivities with p-OH methamphetamine and amphetamine (metabolites of methamphetamine). The range of methamphetamine measurable by the enzyme immunoassay was 1 to 300 ng/tube. According to the assay, methamphetamine could be detected from urine and extract of hair.

摘要

开发了一种用于检测甲基苯丙胺的竞争性酶免疫测定法，该方法以碱性磷酸酶标记的甲基苯丙胺、琼脂糖抗体和对硝基苯磷酸酯作为底物。用N-（4-氨基丁基）甲基苯丙胺-BSA偶联物免疫兔子产生的抗甲基苯丙胺抗血清对甲基苯丙胺具有特异性，与对羟基甲基苯丙胺和苯丙胺（甲基苯丙胺的代谢物）的交叉反应性较低。酶免疫测定法可检测的甲基苯丙胺范围为1至300 ng/管。根据该测定法，可从尿液和毛发提取物中检测出甲基苯丙胺。