[来自链霉菌771的蛋白水解酶制剂的等电聚焦]

[Isoelectric focusing of a preparation of proteolytic enzymes from Streptomyces 771].

作者信息

Krest'ianova I N, Vasil'eva L I, Bartoshevich Iu E, Akparov V Kh, Nakhapetian L A

出版信息

Prikl Biokhim Mikrobiol. 1983 Mar-Apr;19(2):217-25.

Abstract

The proteolytic enzymes contained in the preparation from Streptomyces 771 have been separated by isoelectric focusing in the sucrose density gradient at pH 3-10. The following enzymes have been identified: three multiple forms of neutral metal proteinase (pI 5.1, 6.37, 7.8) each of which splits DNP-Gly-Gly decreases-Val-ArgOMe; elastase-like metal proteinase active with respect to RBB-elestin with pI 10.68; metal-dependent peptidases: leucin aminopeptidase active with respect to L-RBB-elastin with pI 10.68 metal-dependent peptidases: leucin aminopeptidase active with respect to L-leucin n-nitroanilide and L-leucin beta-naphtylamide with pI 7.65, 7.15, 6.67, 6.45, 5.7, 5.35, 5.22, 4.83; carboxy peptidase with pI 5.95, 6.37; serine metal-dependent subtilisin-like proteinase active with respect to 2-Ala-Ala-LeupNA, 2-Gly-Gly-LeupNA, 2-Ala-LeupNA; two multiple forms of serine trypsin-like proteinase active with respect to BAEE and BApNA with pI 4.35, 4.76; serine chymotrypsin-like proteinase with pI 8.68 active with respect to ATEE.

摘要

链霉菌771制备物中所含的蛋白水解酶已通过在pH 3 - 10的蔗糖密度梯度中进行等电聚焦分离。已鉴定出以下几种酶：三种中性金属蛋白酶的多种形式（pI 5.1、6.37、7.8），每种都能裂解DNP - Gly - Gly - 缬氨酸 - 精氨酸 - 甲酯；对RBB - 弹性蛋白有活性的类弹性蛋白酶金属蛋白酶，pI为10.68；金属依赖性肽酶：对L - RBB - 弹性蛋白有活性的亮氨酸氨肽酶，pI为10.68；金属依赖性肽酶：对L - 亮氨酸 - 对硝基苯胺和L - 亮氨酸 - β - 萘酰胺有活性的亮氨酸氨肽酶，pI为7.65、7.15、6.67、6.45、5.7、5.35、5.22、4.83；羧肽酶，pI为5.95、6.37；对2 - Ala - Ala - Leu - pNA、2 - Gly - Gly - Leu - pNA、2 - Ala - Leu - pNA有活性的丝氨酸金属依赖性枯草杆菌蛋白酶样蛋白酶；两种对BAEE和BApNA有活性的丝氨酸胰蛋白酶样蛋白酶的多种形式，pI为4.35、4.76；对ATEE有活性的pI为8.68的丝氨酸胰凝乳蛋白酶样蛋白酶。