[Instrumentation technology problems in immunofluorescence microscopy].

The influence of construction units of the microscope such as filters, lamps and objectives upon fluorescent intensity and contrast is demonstrated. Further, the bleaching of fluorescence and the possibilities of contrast illumination are dealth with. Of crucial importance to the evaluation of the fluorescent images is their contrast rather than the intensity of fluorescence. Narrow band excitation is beneficial and brightening of the background, strong bleaching as well as too great a secondary magnification are disadvantageous to the contrast.