Srebreva L, Zlatanova J
Biochim Biophys Acta. 1983 Jun 24;740(2):163-8. doi: 10.1016/0167-4781(83)90073-8.
The lysine-rich histones of trout liver were studied in order to see whether a protein similar to mammalian histone H1(0) exists in this lower vertebrate. The biochemical methods used involved SDS and acid-urea polyacrylamide gel electrophoresis, gel exclusion and ion-exchange chromatography. Specific rabbit antibodies were elicited against chromatographically-purified mouse liver H1(0) and applied for further characterization of the presumptive trout-liver H1(0) fraction. The results from the indirect immunofluorescence on nitrocellulose-immobilized crude H1 subfractions and from the complement fixation test supplied additional evidence for the existence of an H1(0)-like protein in trout liver.
为了探究在这种低等脊椎动物中是否存在类似于哺乳动物组蛋白H1(0)的蛋白质，对鳟鱼肝脏中富含赖氨酸的组蛋白进行了研究。所采用的生化方法包括SDS和酸性尿素聚丙烯酰胺凝胶电泳、凝胶排阻色谱和离子交换色谱。用经色谱纯化的小鼠肝脏H1(0)诱导产生特异性兔抗体，并用于对推测的鳟鱼肝脏H1(0)组分进行进一步表征。对固定在硝酸纤维素上的粗H1亚组分进行间接免疫荧光检测以及补体结合试验的结果，为鳟鱼肝脏中存在类H1(0)蛋白提供了额外证据。
