Srebreva L N, Andreeva N B, Gasaryan K G, Tsanev R G, Zlatanova J S
Differentiation. 1983;25(2):113-20. doi: 10.1111/j.1432-0436.1984.tb01347.x.
The lysine-rich histones of chicken liver were studied in order to see whether a protein similar to mammalian histone H1o was present in this lower vertebrate. The following biochemical methods were used: sodium dodecylsulphate and acid-urea electrophoresis, gel exclusion chromatography on BioGel P100, and ion-exchange chromatography on BioRex 70. Specific polyclonal antibodies were elicited against purified mouse liver H1o and chicken erythrocyte H5, and applied for the further characterization of the chicken H1 subfractions obtained chromatographically. The results from microcomplement fixation and enzyme-linked immunosorbent assays showed that the presumptive chicken liver H1o shared common antigenic determinants with the mammalian H1o and the chicken liver H5. Based on the combined biochemical and immunological evidence, we conclude that an H1o-like protein is present in quiescent differentiated avian cells. The data of Smith et al. [34], who did not find this specific lysine-rich histone in resting chicken cells, are discussed.
为了探究在这种低等脊椎动物中是否存在与哺乳动物组蛋白H1o相似的蛋白质，研究人员对鸡肝中富含赖氨酸的组蛋白进行了研究。采用了以下生化方法：十二烷基硫酸钠和酸性尿素电泳、在BioGel P100上进行凝胶排阻色谱以及在BioRex 70上进行离子交换色谱。针对纯化的小鼠肝H1o和鸡红细胞H5制备了特异性多克隆抗体，并用于对通过色谱法获得的鸡H1亚组分进行进一步表征。微量补体结合试验和酶联免疫吸附测定的结果表明，推测的鸡肝H1o与哺乳动物H1o和鸡肝H5具有共同的抗原决定簇。基于生化和免疫学证据，我们得出结论，在静止分化的禽类细胞中存在一种类似H1o的蛋白质。文中还讨论了史密斯等人[34]的数据，他们在静止的鸡细胞中未发现这种特异性富含赖氨酸的组蛋白。
