IS50 直接重复序列间不依赖 RecA 的重组

RecA-independent recombination between direct repeats of IS50.

作者信息

Zupancic T J, Marvo S L, Chung J H, Peralta E G, Jaskunas S R

出版信息

Cell. 1983 Jun;33(2):629-37. doi: 10.1016/0092-8674(83)90444-0.

Abstract

Recombination between the IS50 sequences that flank Tn5 has been found to occur readily after the transformation of E. coli recA strains with a plasmid that contains direct repeats of these sequences. Analysis of mutants of IS50 indicates that the polypeptide encoded by IS50 that is required for transposition is also required for the recombination. Surprisingly, the mechanism of recombination appears to be similar to general homologous recombination rather than site-specific recombination. This IS50 recombination system may be responsible for resolving transient cointegrate structures containing direct repeats of IS50 or Tn5 during the transposition of IS50 and Tn5.

摘要

在用含有这些序列直接重复序列的质粒转化大肠杆菌recA菌株后，已发现位于Tn5两侧的IS50序列之间很容易发生重组。对IS50突变体的分析表明，转座所需的由IS50编码的多肽在重组中也是必需的。令人惊讶的是，重组机制似乎类似于一般的同源重组，而不是位点特异性重组。这种IS50重组系统可能负责在IS50和Tn5转座过程中解析包含IS50或Tn5直接重复序列的瞬时共整合结构。