基因49参与噬菌体T4的重组。
Involvement of gene 49 in recombination of bacteriophage T4.
作者信息
Miyazaki J, Ryo Y, Minagawa T
出版信息
Genetics. 1983 May;104(1):1-9. doi: 10.1093/genetics/104.1.1.
The role of T4 gene 49 in recombination was investigated using its conditional-lethal amber (am) and temperature-sensitive (ts) mutants. When measured in genetic tests, defects in gene 49 produced a recombination-deficient phenotype. However, DNA synthesized in cells infected with a ts mutant (tsC9) at a nonpermissive temperature appeared to be in a recombinogenic state: after restitution of gene function by shifting to a permissive temperature, the recombinant frequency among progeny increased rapidly even when DNA replication was blocked by an inhibitor. Growth of a gene 49-defective mutant was suppressed by an additional mutation in gene uvsX, but recombination between rII markers was not.
利用T4噬菌体基因49的条件致死琥珀型（am）和温度敏感型（ts）突变体研究了其在重组中的作用。在遗传学检测中，基因49的缺陷产生了重组缺陷表型。然而，在非允许温度下用ts突变体（tsC9）感染的细胞中合成的DNA似乎处于重组状态：通过转移到允许温度恢复基因功能后，即使DNA复制被抑制剂阻断，子代中的重组频率也会迅速增加。基因49缺陷型突变体的生长受到uvsX基因额外突变的抑制，但rII标记之间的重组不受影响。
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