Barnett M L, Baker R L
J Periodontol. 1983 May;54(5):272-6. doi: 10.1902/jop.1983.54.5.272.
The Application of electron microscopic methods to the study of neutrophils obtained from single sites by crevicular washing is described. A subsequent preliminary study of neutrophils and neutrophil-bacterial interactions at advanced lesions in patients with juvenile periodontitis and juvenile diabetes indicated that the neutrophils are phagocytically active at these sites. It is suggested that in vitro assays of crevicular neutrophil function may exaggerate the extent of impairment of phagocytosis because of cellular activity which has taken place prior to the time of sampling.
本文描述了电子显微镜方法在研究通过龈沟冲洗从单个部位获取的中性粒细胞中的应用。随后对青少年牙周炎和青少年糖尿病患者晚期病变处中性粒细胞及中性粒细胞与细菌相互作用的初步研究表明，这些部位的中性粒细胞具有吞噬活性。有人提出，由于在采样前已经发生了细胞活动，龈沟中性粒细胞功能的体外检测可能会夸大吞噬作用受损的程度。
