结合反应对芳香胺致突变性的影响。

Influence of conjugation reactions on the mutagenicity of aromatic amines.

作者信息

Hongslo J, Haug L T, Wirth P J, Møller M, Dybing E, Thorgeirsson S S

出版信息

Mutat Res. 1983 Feb;107(2):239-47. doi: 10.1016/0027-5107(83)90166-5.

Abstract

2-Acetylaminofluorene (AAF) and 2-aminofluorene (AF), as well as their N-hydroxylated metabolites, N-OH-AAF and N-OH-AF, were studied for mutagenic effects in Salmonella typhimurium with rat- and mouse-liver S9 and microsomal subfractions in the presence of cofactors for glucuronidation and glutathione (GSH) transfer. Addition of UDPGA did not affect the mutagenicity of AAF, AF or N-OH-AAF under any experimental condition. Addition of GSH, on the other hand, markedly inhibited AAF, AF and N-OH-AAF. This seemed to be due to the direct effect of GSH, and not through an enzyme-catalyzed conjugation. Further, GSH inhibited the direct mutagenicity of N-OH-AF.

摘要

研究了2-乙酰氨基芴（AAF）、2-氨基芴（AF）及其N-羟基化代谢产物N-OH-AAF和N-OH-AF在鼠伤寒沙门氏菌中的致突变作用，使用大鼠和小鼠肝脏S9及微粒体亚组分，并存在葡糖醛酸化和谷胱甘肽（GSH）转移的辅因子。在任何实验条件下，添加尿苷二磷酸葡糖醛酸（UDPGA）均不影响AAF、AF或N-OH-AAF的致突变性。另一方面，添加GSH可显著抑制AAF、AF和N-OH-AAF。这似乎是由于GSH的直接作用，而非通过酶催化结合。此外，GSH抑制N-OH-AF的直接致突变性。