Everett A W, Prior G, Clark W A, Zak R
Anal Biochem. 1983 Apr 1;130(1):102-7. doi: 10.1016/0003-2697(83)90655-3.
The amount of myosin per gram of cardiac and skeletal muscle was determined in sodium dodecyl sulfate-solubilized tissue homogenates by radioimmunoassay and by isotope dilution. In the rabbit ventricle, there was an average of 27 mg myosin/g wet wt of tissue. In chickens, the myosin content of typical "red" (anterior latissimus dorsi) and "white" (posterior latissimus dorsi) skeletal muscles was higher than that of ventricular muscle, averaging 36 and 48 mg/g of tissue, respectively. The stoichiometry of the heavy and light chains in cardiac myosin was also determined from the quantitative binding of 125I-labeled Coomassie blue to each subunit after separation of the subunits by sodium dodecyl sulfate-gel electrophoresis. With this procedure, we found that the combined light-chain subunits contributed 19% of the myosin mass. After adjustment for the light-chain contribution, the myosin heavy-chain content of the rabbit ventricle averaged 22 mg/g wet wt of tissue.
通过放射免疫测定法和同位素稀释法，测定了十二烷基硫酸钠溶解的组织匀浆中每克心肌和骨骼肌的肌球蛋白含量。在兔心室中，每克湿重组织平均含有27毫克肌球蛋白。在鸡中，典型的“红色”（背阔肌前部）和“白色”（背阔肌后部）骨骼肌的肌球蛋白含量高于心室肌，分别平均为每克组织36毫克和48毫克。通过十二烷基硫酸钠-凝胶电泳分离亚基后，利用125I标记的考马斯亮蓝与每个亚基的定量结合，还测定了心肌肌球蛋白中重链和轻链的化学计量。通过该方法，我们发现轻链亚基的总和占肌球蛋白质量的19%。在对轻链贡献进行调整后，兔心室肌球蛋白重链含量平均为每克湿重组织22毫克。
