兔骨骼肌中肌球蛋白和肌动蛋白的定量测定。

Quantitative determination of myosin and actin in rabbit skeletal muscle.

作者信息

Yates L D, Greaser M L

出版信息

J Mol Biol. 1983 Jul 25;168(1):123-41. doi: 10.1016/s0022-2836(83)80326-x.

Abstract

The myosin and actin content of muscle tissue and purified myofibrils from rabbit psoas muscle has been determined. Myofibrils were purified using Percoll gradients, which allowed rapid separation from nuclei and connective tissue proteins. Myosin and actin were quantitated by amino acid analysis of the appropriate bands from sodium dodecyl sulfate/polyacrylamide gels. Muscle tissue contained 94 and 619 nmol/g wet weight of myosin and actin, respectively, while myofibrils had 0.82 and 5.37 mumol/g protein. Thus myosin contributed 43% and actin 22% of the myofibril protein mass. The value of 2.5 myosins per 14.3 nm repeat as calculated from these results suggests that thick filament models with mixtures of two and three crossbridges per repeat should be considered.

摘要

已测定兔腰大肌肌肉组织和纯化肌原纤维中的肌球蛋白和肌动蛋白含量。使用Percoll梯度纯化肌原纤维，该方法可快速从细胞核和结缔组织蛋白中分离出来。通过对十二烷基硫酸钠/聚丙烯酰胺凝胶中相应条带进行氨基酸分析来定量肌球蛋白和肌动蛋白。肌肉组织中肌球蛋白和肌动蛋白的含量分别为94和619 nmol/g湿重，而肌原纤维中分别为0.82和5.37 μmol/g蛋白质。因此，肌球蛋白占肌原纤维蛋白质质量的43%，肌动蛋白占22%。根据这些结果计算出每14.3 nm重复中有2.5个肌球蛋白，这表明应考虑每重复有两个和三个横桥混合物的粗丝模型。