Konttinen Y T, Tolvanen E, Visa-Tolvanen K, Reitamo S, Förström L
Clin Immunol Immunopathol. 1983 Mar;26(3):380-9. doi: 10.1016/0090-1229(83)90122-8.
Inflammatory cells in situ in Kveim reaction papules were identified in 15 patients with avidin-biotin-peroxidase complex and biotin-avidin-peroxidase methods for surface epitopes and with a simultaneously capturing azo dye method for cytoplasmic acid alpha-naphthyl acetate esterase (ANAE). The spatial relationship of cells in granulomas indicates a concentric arrangement. Endogenous peroxidase-negative, ANAE+, but OKIa-negative immunoincompetent epithelioid cells were in the center. T3+, ANAE+ T lymphocytes formed 60-80% of all cells in the lymphocyte mantle surrounding the epithelioid core. T4/T8 was 2:1. Equal proportions (5-15%) of Ia+ lymphocytes and M pattern ANAE+, endogenous peroxidase-positive mononuclear phagocytes on the one hand and T3+ and T pattern ANAE+ cells on the other in individual patients indicate that the proportion of activated T blasts in situ was less than 15%. The close contacts between different immunocompetent cells in the periphery indicates this as the site for cellular interactions.
采用抗生物素蛋白-生物素-过氧化物酶复合物法和生物素-抗生物素蛋白-过氧化物酶法检测表面抗原决定簇，并同时采用捕获偶氮染料法检测细胞质酸性α-萘乙酸酯酶（ANAE），在15例患者的克维姆反应丘疹中发现了原位炎症细胞。肉芽肿中细胞的空间关系呈同心排列。内源性过氧化物酶阴性、ANAE阳性但OKIa阴性的无免疫活性上皮样细胞位于中心。T3阳性、ANAE阳性的T淋巴细胞占围绕上皮样核心的淋巴细胞套中所有细胞的60% - 80%。T4/T8为2:1。在个体患者中，一方面Ia阳性淋巴细胞和M型ANAE阳性、内源性过氧化物酶阳性的单核吞噬细胞比例相当（5% - 15%），另一方面T3阳性和T型ANAE阳性细胞比例相当，这表明原位活化T母细胞的比例小于15%。外周不同免疫活性细胞之间的紧密接触表明此处为细胞相互作用的部位。
