Lie T S, Galkowska H, Jaeger K, Niehaus K J
Langenbecks Arch Chir. 1983;360(1):17-27. doi: 10.1007/BF01255580.
To investigate the immunological status of hepatic grafts we transplanted livers from different inbred rats orthotopically on 58 LEW rats; rearterialization of the grafts was achieved with an hepatico aortal segment of the donor. Survival time depended on the donor strain; LBNF1-allografts survived in 67% (n = 12), WiS- in 60% (n = 20) and BUF- in 43% (n = 14) indefinitely. In the DA-to-LEW-combination we found no long-term survivor. Liver perfusates (LP) were prepared from 10 BDE and 10 LEW; after keeping the livers for 6 h at room temperature we perfused via portal vein with 2 ml/g Ringer's solution five times. The treatment of BDE-kidney recipients (LEW) with LP showed prolonged survival; at 5 days application 10.2 +/- 1.3 d (control: 6.5 +/- 0.5 d; P less than 0.001), at 10 days treatment 15.3 +/- 7.3 d. In vitro LP inhibited the PHA-stimulation of LEW lymphoid cells in more than 90% and the ConA-stimulation of LEW spleen cells in more than 95%. In MLC LP showed strong inhibitory effect (inhibition rate greater than 97%) even when different combinations of responding and stimulating cells were used. We assume that an unspecific immunosuppressive hepatic factor is released from ischemic damaged liver grafts which is able to prevent rejection in the induction phase. In WiS-liver recipients surviving for more than 4 months GvHR was tested after splenectomy with spleen cells. All tests showed a grade III reaction. Donor-specific skin grafts which were transplanted on these recipients survived indefinitely while third party skin grafts were regularly rejected (7.6 +/- 0.5 d). We therefore can conclude that the cellular immunosurveillance is intact, but the immunological response against donor-specific antigens is reduced. With transfer of serum from long-term-surviving WiS-liver recipients (greater than 6 months) WiS-kidney grafted LEW were able to survive also prolonged (20.7 +/- 3.4 d, control 6.2 +/- 1.0 d; P less than 0.001). Lymphoid cell transfer (1 X 10(8)) did did not result in significant prolongation of survival time (8.0 +/- 2.0 d). These observations suggest that in the steady phase specific humoral transfer factors are responsible for prolonged survival of hepatic grafts.
为研究肝移植的免疫状态，我们将不同近交系大鼠的肝脏原位移植到58只LEW大鼠上；通过供体的肝主动脉段实现移植肝的再动脉化。存活时间取决于供体品系；LBNF1同种异体移植物的长期存活率为67%（n = 12），WiS为60%（n = 20），BUF为43%（n = 14）。在DA对LEW的组合中，我们未发现长期存活者。从10只BDE和10只LEW大鼠制备肝灌注液（LP）；将肝脏在室温下保存6小时后，经门静脉用2 ml/g林格氏液灌注5次。用LP处理BDE肾移植受体（LEW）可延长存活时间；在应用5天时为10.2±1.3天（对照组：6.5±0.5天；P＜0.001），在应用10天时为15.3±7.3天。体外LP抑制LEW淋巴细胞对PHA的刺激超过90%，抑制LEW脾细胞对ConA的刺激超过95%。在混合淋巴细胞培养中，即使使用不同组合的反应细胞和刺激细胞，LP也显示出强烈的抑制作用（抑制率＞97%）。我们推测，缺血损伤的肝移植物释放出一种非特异性免疫抑制肝因子，它能够在诱导期预防排斥反应。在存活超过4个月的WiS肝移植受体中，脾切除后用脾细胞检测移植物抗宿主反应（GvHR）。所有检测均显示为III级反应。移植到这些受体上的供体特异性皮肤移植物长期存活，而第三方皮肤移植物则被定期排斥（7.6±0.5天）。因此，我们可以得出结论，细胞免疫监视功能完好，但针对供体特异性抗原的免疫反应降低。将长期存活（＞6个月）的WiS肝移植受体的血清转移后，移植了WiS肾的LEW大鼠也能延长存活时间（20.7±3.4天，对照组6.2±1.0天；P＜0.001）。淋巴细胞转移（1×10⁸）并未导致存活时间显著延长（8.0±2.0天）。这些观察结果表明，在稳定期，特异性体液转移因子是肝移植物长期存活的原因。
