大肠杆菌中RNA聚合酶利福平抗性突变：序列变化与显性
RNA polymerase rifampicin resistance mutations in Escherichia coli: sequence changes and dominance.
作者信息
Ovchinnikov Y A, Monastyrskaya G S, Guriev S O, Kalinina N F, Sverdlov E D, Gragerov A I, Bass I A, Kiver I F, Moiseyeva E P, Igumnov V N, Mindlin S Z, Nikiforov V G, Khesin R B
出版信息
Mol Gen Genet. 1983;190(2):344-8. doi: 10.1007/BF00330662.
Five recombinant plasmids, pBK2646, pBK611, pRC3, pRC4 and pRC5, carrying rpoB rifampicin-resistant RNA-polymerase genes were obtained. The sequence analysis of these plasmids revealed certain structural changes in the rpoB gene which specify corresponding alterations in the beta-subunit of RNA polymerase. Some functional properties of the corresponding mutant strains and their RNA polymerases have been investigated.
获得了携带rpoB利福平抗性RNA聚合酶基因的5种重组质粒，即pBK2646、pBK611、pRC3、pRC4和pRC5。对这些质粒的序列分析揭示了rpoB基因中的某些结构变化，这些变化决定了RNA聚合酶β亚基的相应改变。已经研究了相应突变菌株及其RNA聚合酶的一些功能特性。
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