侵袭内阿米巴核糖体的初步特征分析。

Preliminary characterization of ribosomes of Entamoeba invadens.

作者信息

Price M, Specht C, Boudreau R E, Shaw D E, Weller D L

出版信息

Mol Biochem Parasitol. 1983 Jun;8(2):137-43. doi: 10.1016/0166-6851(83)90005-1.

Abstract

The ribosomes of Entamoeba invadens trophozoites have sedimentation coefficients of 77, 53 and 36 S. Most of the ribosomal proteins are basic and their one- and two-dimensional electrophoretic patterns differ from the corresponding patterns of Escherichia coli and Saccharomyces cerevisiae. Two dozen bands were observed in the 10 000 to 100 000 molecular weight range following sodium dodecylsulfate-gel electrophoresis of amoebal ribosomal proteins. Long, thin pronase-sensitive structures were seen in electron micrographs of E. invadens ribosomal preparations.

摘要

侵袭内阿米巴滋养体的核糖体沉降系数为77、53和36 S。大多数核糖体蛋白呈碱性，其一维和二维电泳图谱与大肠杆菌和酿酒酵母的相应图谱不同。对变形虫核糖体蛋白进行十二烷基硫酸钠凝胶电泳后，在10000至100000分子量范围内观察到二十多条条带。在侵袭内阿米巴核糖体制剂的电子显微照片中可见长而细的对链霉蛋白酶敏感的结构。