质粒DNA的大规模纯化
Large scale purification of plasmid DNA.
作者信息
Bachvarov D R, Ivanov I G
出版信息
Prep Biochem. 1983;13(2):161-6. doi: 10.1080/00327488308068745.
PMID:6348728
Abstract
A simple and rapid procedure for large scale purification of plasmid DNA is described. The procedure consists of two main steps: 1. Alkaline extraction of plasmid DNA (by a slight modification of the method of Birnboim and Doly (1)) and 2. Purification of the crude extract by hydroxyapatite chromatography. The plasmids obtained are biologically active and can be used in gene manipulation experiments.
摘要
本文描述了一种用于大规模纯化质粒DNA的简单快速方法。该方法包括两个主要步骤：1. 质粒DNA的碱提取（对Birnboim和Doly(1)的方法进行了轻微修改）和2. 通过羟基磷灰石色谱法纯化粗提物。所获得的质粒具有生物活性，可用于基因操作实验。
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