Tufveson G, Forsum U, Claesson K, Klareskog L, Larsson E, Karlsson-Parra A, Frödin L
Scand J Immunol. 1983 Jul;18(1):37-40. doi: 10.1111/j.1365-3083.1983.tb00833.x.
This communication describes an immunohistochemical analysis of rejected human renal allografts. T-lymphocyte subsets were identified in frozen tissue sections, by Leu 1 (anti-'pan' T lymphocytes), Leu 2a (anti-'cytotoxic/suppressor' T cells), and Leu 3a (anti-'helper/inducer' T cells) monoclonal antibodies. In addition, HLA-DR-positive cells were identified by simultaneous labelling with heterologous anti-HLA-DR antibodies. T cells dominated the cellular infiltrates in acute cellular rejection. Leu-3a-positive cells were more numerous than Leu-2a-positive cells. The Leu-3a-positive cells usually appeared in clusters, whereas the Leu-2a-positive cells appeared scattered in the tissue. HLA-DR-positive non-T cells were found within clusters of T 'helper/inducer' cells. The cell pattern shares many features with the findings in delayed-type hypersensitivity reactions.
本通讯描述了对人同种异体移植肾排斥反应的免疫组织化学分析。通过Leu 1（抗“全”T淋巴细胞）、Leu 2a（抗“细胞毒性/抑制性”T细胞）和Leu 3a（抗“辅助/诱导性”T细胞）单克隆抗体，在冷冻组织切片中鉴定T淋巴细胞亚群。此外，通过与异源抗HLA-DR抗体同时标记来鉴定HLA-DR阳性细胞。在急性细胞排斥反应中，T细胞在细胞浸润中占主导地位。Leu-3a阳性细胞比Leu-2a阳性细胞数量更多。Leu-3a阳性细胞通常成簇出现，而Leu-2a阳性细胞则分散在组织中。在T“辅助/诱导性”细胞簇中发现了HLA-DR阳性非T细胞。这种细胞模式与迟发型超敏反应的发现有许多共同特征。
