Sinha S K, Yunis A A
Biochem Biophys Res Commun. 1983 Jul 29;114(2):797-803. doi: 10.1016/0006-291x(83)90852-5.
Human milk contains colony stimulating factor (CSF), a polypeptide growth factor, which stimulates in in vitro bone marrow culture proliferation and differentiation of colony forming granulocytic macrophage progenitor cells (CFU-GM) to form colonies. This activity was not found in either bovine milk or colostrum when assayed in human or mouse bone marrow cells. The human milk CSF activity is destroyed by treatment with proteases. However, neither 6M urea, 4M guanidine hydrochloride, 5 mM dithiothreitol, nor exposure to pH 2 will inactivate the milk derived CSF. Gel filtration and isoelectric focusing indicate that human milk CSF differs biochemically from the other CSFs isolated from various sources and has a molecular weight between 250,000 and 240,000 and an isoelectric point between 4.4 and 4.9.
人乳中含有集落刺激因子（CSF），一种多肽生长因子，它在体外骨髓培养中能刺激集落形成粒细胞巨噬细胞祖细胞（CFU-GM）的增殖和分化，从而形成集落。当用人或小鼠骨髓细胞进行检测时，无论是牛乳还是初乳中都未发现这种活性。人乳CSF活性经蛋白酶处理后会被破坏。然而，6M尿素、4M盐酸胍、5mM二硫苏糖醇或暴露于pH 2环境均不会使源自乳汁的CSF失活。凝胶过滤和等电聚焦表明，人乳CSF在生化性质上与从各种来源分离的其他CSF不同，其分子量在250,000至240,000之间，等电点在4.4至4.9之间。
