White F H, Gohari K
J Anat. 1983 Jun;136(Pt 4):801-19.
The present report describes quantitative studies of mitochondria from stratified squamous epithelial cells from hamster cheek pouch mucosa in differentiating compartments. Mucosa was processed for electron microscopy and, using strict sampling procedures, electron micrographs of epithelium from defined basal, spinous and granular strata were acquired. Using stereological and other morphometric methods, the volume density, numerical density and volume-to-surface ratio of mitochondria were estimated in each cellular layer, and these data were transformed into absolute values characterising the average cell in these layers. The results suggest that both volumetric and numerical densities of mitochondria decreased progressively during epithelial differentiation; conversely, the total volume and number of mitochondria present in granular cells were consistently higher than in basal cells, as was the volume of individual mitochondria. These quantitative changes may reflect functional alterations in the metabolism of epithelial cells during differentiation and provide a base line for further work on experimental epithelial biology and pathology.
本报告描述了对来自仓鼠颊囊黏膜分层鳞状上皮细胞不同分化区室中线粒体的定量研究。对黏膜进行电子显微镜处理，并采用严格的采样程序，获取了来自明确的基底、棘状和颗粒层上皮的电子显微照片。使用体视学和其他形态测量方法，估计了每个细胞层中线粒体的体积密度、数量密度和体积与表面积比，并将这些数据转换为表征这些层中平均细胞的绝对值。结果表明，在上皮分化过程中，线粒体的体积密度和数量密度均逐渐降低；相反，颗粒细胞中线粒体的总体积和数量一直高于基底细胞，单个线粒体的体积也是如此。这些定量变化可能反映了上皮细胞分化过程中代谢的功能改变，并为进一步开展实验性上皮生物学和病理学研究提供了基线。
