一种新的灵敏且便捷的鸟氨酸转氨酶检测方法。

A new sensitive and convenient assay of ornithine aminotransferase.

作者信息

Ohura T, Kominami E, Katunuma N

出版信息

J Nutr Sci Vitaminol (Tokyo). 1983 Apr;29(2):123-8. doi: 10.3177/jnsv.29.123.

DOI:10.3177/jnsv.29.123

Abstract

A new radioisotopic assay for ornithine aminotransferase with [U-14C]ornithine as a substrate was developed. The dihydroquinazolinium compound formed was extracted from the reaction mixture with n-butanol and an addition of high concentration of sodium sulfate. This assay is sensitive and convenient and more than 50 assays can be done in one day without any special apparatus. The activities of ornithine aminotransferase in rat liver and human lymphoblastoid cells could be measured at nanomole levels by this method. Thus, the assay should be useful for measurement of OAT activity in cultured cells and for studies on the metabolic bases of the different types of ornithine aminotransferase deficiency: vitamin B6-responsive and -nonresponsive types.

摘要

开发了一种以[U-14C]鸟氨酸为底物的鸟氨酸氨基转移酶放射性同位素新测定法。反应混合物中形成的二氢喹唑啉化合物用正丁醇并添加高浓度硫酸钠进行萃取。该测定法灵敏且方便，无需任何特殊仪器，一天内可进行50多次测定。用此方法可在纳摩尔水平测定大鼠肝脏和人淋巴母细胞中鸟氨酸氨基转移酶的活性。因此，该测定法对于测量培养细胞中的鸟氨酸氨基转移酶活性以及研究不同类型鸟氨酸氨基转移酶缺乏症（维生素B6反应型和非反应型）的代谢基础应是有用的。