Bartnik E, de Robertis E M
J Mol Biol. 1983 Aug 5;168(2):439-44. doi: 10.1016/s0022-2836(83)80027-8.
Three plasmids containing Aspergillus nidulans mitochondrial transfer RNA genes were microinjected into the nucleus of Xenopus laevis oocytes. Plasmids that contained a single tRNACys gene or two tRNA genes (Arg and Asn) yielded tRNA-sized transcripts. Plasmids containing cloned Lupinus mitochondrial tRNA genes were also transcribed and processed in X. laevis nuclei.
将三个含有构巢曲霉线粒体转移RNA基因的质粒显微注射到非洲爪蟾卵母细胞的细胞核中。含有单个tRNACys基因或两个tRNA基因(Arg和Asn)的质粒产生了tRNA大小的转录本。含有克隆的羽扇豆线粒体tRNA基因的质粒也在非洲爪蟾细胞核中进行转录和加工。