Cumming R, Burgoyne R D, Lytton N A, Gray E G
Neurosci Lett. 1983 Jun 30;37(3):215-20. doi: 10.1016/0304-3940(83)90433-0.
Ultrastructural studies of intact tissue rarely show presynaptic microtubules, and immunocytochemical studies on tissue sections have previously been unable to demonstrate tubulin in the nerve terminal. In contrast, a microtubular coil can be readily detected in the presynaptic nerve terminal of synaptosomes. We have developed an immunocytochemical procedure on the synaptosome preparation and demonstrated, using monoclonal antibodies, that in the presynaptic terminal alpha and beta tubulin subunits are specifically restricted to the equatorial microtubular coil.
对完整组织的超微结构研究很少能显示突触前微管，并且之前对组织切片的免疫细胞化学研究未能在神经末梢中证实微管蛋白的存在。相比之下，在突触体的突触前神经末梢中可以很容易地检测到微管卷曲。我们开发了一种针对突触体制备的免疫细胞化学方法，并使用单克隆抗体证明，在突触前末梢，α和β微管蛋白亚基特异性地局限于赤道微管卷曲处。
