Khristin M S
Biokhimiia. 1983 Jul;48(7):1067-73.
The effects of redox conversions of plastocyanin copper chromophore on the formation of plastocyanin complexes with cytochrome f and the reaction center of photosystem I from pea chloroplasts were studied. In order to investigate the complex formation plastocyanin and cytochrome f were immobilized on Sephadex G-200. The cytochrome f and reaction center assembly takes place on the immobilized plastocyanin, which is necessary for cytochrome f photooxidation. It was found that in a reconstituted system the reduced plastocyanin forms more stable complexes with the proteins than the oxidized one, which is due to its lower pI value.
研究了质体蓝素铜发色团的氧化还原转化对质体蓝素与细胞色素f以及豌豆叶绿体光系统I反应中心形成复合物的影响。为了研究复合物的形成，将质体蓝素和细胞色素f固定在葡聚糖凝胶G - 200上。细胞色素f和反应中心组件在固定化的质体蓝素上发生组装，这是细胞色素f光氧化所必需的。结果发现，在重构系统中，还原型质体蓝素比氧化型质体蓝素与蛋白质形成更稳定的复合物，这是由于其较低的等电点值。
