一种分子量为45000的人肾素前体在无细胞翻译系统中合成。

A 45 000 molecular weight human renin precursor is synthesized in a cell-free translation system.

作者信息

Parmentier M, Inagami T, Pochet R

出版信息

Clin Sci (Lond). 1983 Nov;65(5):475-7. doi: 10.1042/cs0650475.

Abstract

Human kidney mRNA species were isolated and fractionated through a continuous sucrose gradient ultracentrifugation. mRNA fractions were translated by using a rabbit reticulocyte lysate and [35S]methionine as tracer. Double immunoprecipitation was carried out with highly specific anti-human renin and anti-rabbit gamma-globulin antisera. A 15S mRNA has been found to direct synthesis of a 45 000 molecular weight protein immunoprecipitable with anti-human renin. This protein is considered to be the ultimate precursor of renin (pre-prorenin).

摘要

通过连续蔗糖梯度超速离心法分离并分级分离人肾信使核糖核酸（mRNA）种类。使用兔网织红细胞裂解物和[35S]甲硫氨酸作为示踪剂对mRNA级分进行翻译。用高度特异性的抗人肾素和抗兔γ-球蛋白抗血清进行双重免疫沉淀。已发现一种15S的mRNA可指导合成一种分子量为45000的蛋白质，该蛋白质可被抗人肾素免疫沉淀。这种蛋白质被认为是肾素的最终前体（前肾素原）。