Young M F, Klein N W
Dev Biol. 1983 Nov;100(1):50-8. doi: 10.1016/0012-1606(83)90199-9.
Endodermal cells were isolated from yolk sacs of 3-day chick embryos and cultured for 6 days in Eagle's minimal essential media plus 10% fetal calf serum. During this period cells rapidly lost their ability to synthesize DNA as judged by [3H]thymidine incorporation into DNA. In spite of this loss of DNA synthesis serum protein synthesis and secretion remained at a constant 45% of total protein synthesis and secretion. This was determined by immunoprecipitation of culture media using antibodies directed against embryonic chick serum proteins. Media were also analyzed for the synthesis and secretion of specific serum proteins using polyacrylamide gel electrophoresis. The relative synthesis and secretion of the individual serum proteins followed that previously observed in ovo with the exception of alpha-globulin-a which became undetectable. When culture media were supplemented with ovalbumin or insulin the relative synthesis and secretion of certin specific serum proteins were altered. However, analysis of these same media samples showed that the total amounts of serum protein synthesis and secretion were unaffected.
从3日龄鸡胚的卵黄囊中分离出内胚层细胞，并在含有10%胎牛血清的伊格尔基本培养基中培养6天。在此期间，通过[3H]胸腺嘧啶掺入DNA判断，细胞迅速丧失了合成DNA的能力。尽管DNA合成能力丧失，但血清蛋白合成和分泌仍保持在总蛋白合成和分泌的45%不变。这是通过使用针对鸡胚血清蛋白的抗体对培养基进行免疫沉淀来确定的。还使用聚丙烯酰胺凝胶电泳分析培养基中特定血清蛋白的合成和分泌情况。除了无法检测到的α-球蛋白-a外，各个血清蛋白的相对合成和分泌情况与之前在鸡胚内观察到的一致。当向培养基中添加卵清蛋白或胰岛素时，某些特定血清蛋白的相对合成和分泌会发生改变。然而，对这些相同培养基样本的分析表明，血清蛋白合成和分泌的总量未受影响。
