Devine K M, Bergmann J E, Loomis W F
Dev Biol. 1983 Oct;99(2):437-46. doi: 10.1016/0012-1606(83)90293-2.
Previous studies have shown that Dictyostelium discoideum spore coat proteins are found in prespore cells, which are localized to the posterior region of migrating slugs, and in the coats of mature spores. Prespore vesicles, identified by morphology and by staining with anti-D. mucoroides spore serum, are also localized in the posterior region of migrating slugs. Using antisera specific to the spore coat proteins, we show that the spore coat proteins are packaged in prespore vesicles. They are present in the vesicles as a complex which can be dissociated by denaturation. The anti-D. mucoroides spore serum reacts with at least five proteins in whole spore extracts including the spore coat proteins SP96 and SP70.
先前的研究表明，盘基网柄菌的孢子壁蛋白存在于前孢子细胞中，这些细胞定位于迁移蛞蝓的后部区域，也存在于成熟孢子的壁中。通过形态学和用抗毛霉状菌孢子血清染色鉴定的前孢子囊泡也定位于迁移蛞蝓的后部区域。使用针对孢子壁蛋白的抗血清，我们表明孢子壁蛋白被包装在前孢子囊泡中。它们以复合物的形式存在于囊泡中，该复合物可通过变性解离。抗毛霉状菌孢子血清与全孢子提取物中的至少五种蛋白质发生反应，包括孢子壁蛋白SP96和SP70。
