Mandel T E, Georgiou H M
Diabetes. 1983 Oct;32(10):915-20. doi: 10.2337/diab.32.10.915.
Fetal human pancreata, obtained from legally-induced abortions, were placed in organ culture to study their capacity to produce insulin over periods in vitro of between 18 and 40 days. Specimens obtained by hysterotomy usually showed increasing insulin secretion as measured by the insulin content of the media at each twice-weekly medium change. In most instances, relatively little insulin was produced during the first 7-10 days, but thereafter insulin secretion rapidly increased. In contrast, most specimens from prostaglandin-induced abortions showed high levels of insulin in the medium early in the culture period but little thereafter, indicating rapid release of insulin from damaged tissue. In some instances, after early insulin release by damaged tissue, some recovery of islet function occurred and insulin secretion again increased. The presence of differentiated endocrine cells was confirmed histologically. Tissue from each fetus was placed in a number of petri dishes, and for each individual fetus a qualitatively similar pattern of secretion was noted in each dish. These data suggest that representative and nondestructive monitoring of islet function is possible and may be important before such tissue is considered for use in islet transplantation in insulin-dependent diabetes.
从合法引产获取的人类胎儿胰腺被置于器官培养中，以研究其在体外18至40天期间产生胰岛素的能力。通过子宫切开术获得的标本，在每次每两周更换一次培养基时，根据培养基中的胰岛素含量测量，通常显示胰岛素分泌增加。在大多数情况下，最初7至10天产生的胰岛素相对较少，但此后胰岛素分泌迅速增加。相比之下，大多数来自前列腺素引产的标本在培养初期培养基中胰岛素水平较高，但此后则很少，表明胰岛素从受损组织中快速释放。在某些情况下，受损组织早期释放胰岛素后，胰岛功能出现一些恢复，胰岛素分泌再次增加。组织学证实了分化内分泌细胞的存在。每个胎儿的组织被放置在多个培养皿中，对于每个个体胎儿，在每个培养皿中都观察到了定性相似的分泌模式。这些数据表明，在考虑将此类组织用于胰岛素依赖型糖尿病的胰岛移植之前，对胰岛功能进行代表性且非破坏性的监测是可能的，而且可能很重要。
