Grande P, Christiansen C
Scand J Clin Lab Invest. 1983 May;43(3):197-201.
Seven methods commercially available involving different principles for determination of creatine kinase (E.C. 2.7.3.2) isoenzyme MB were compared. The methods employ quantitative electrophoresis, column chromatography, batch chromatography, and antibody inhibition techniques. Blood from 100 patients suspected of acute myocardial infarction was used to test the following factors: sensitivity, linearity, precision, accuracy, simplicity, and diagnostic comparability. The analytical quality of all the techniques investigated was almost satisfactory, and only minor differences were found in linearity and precision. The diagnostic quality was, however, somewhat limited. The number of positive and negative results varied significantly. The reference value given by the manufacturers did not agree, and each laboratory must establish its own reference ranges.
比较了七种市售的、基于不同原理测定肌酸激酶（E.C. 2.7.3.2）同工酶MB的方法。这些方法采用了定量电泳、柱色谱、批量色谱和抗体抑制技术。使用100名疑似急性心肌梗死患者的血液来检测以下因素：灵敏度、线性、精密度、准确度、简易性和诊断可比性。所研究的所有技术的分析质量几乎令人满意，仅在线性和精密度方面发现了微小差异。然而，诊断质量在一定程度上受到限制。阳性和阴性结果的数量差异显著。制造商给出的参考值不一致，每个实验室必须建立自己的参考范围。
