DasGupta B E, Rasmussen S
Toxicon. 1983;21(4):566-9. doi: 10.1016/0041-0101(83)90136-8.
To develop reliable data on the amino acid composition of type F botulinum neurotoxin, three batches of the neurotoxin were analyzed. Each batch was isolated from a separate neurotoxin producing bacterial culture. Two batches had inoculum from one source and the other batch one from a different source. Two batches of the neurotoxin were purified by the same method and one was purified by a different method. The neurotoxin preparations were found comparable in purity and similar in amino acid composition. The best estimate of number of amino acid residues per neurotoxin molecule (mol. wt. 155,000) was: Asp218 Thr80 Ser105 Glu128 Pro47 Gly69 Ala47 Val72 CyS9 Met14 Ile128 Leu104 Tyr86 Phe60 Lys90 His13 Arg51 Trp23.
为了获得关于F型肉毒杆菌神经毒素氨基酸组成的可靠数据，对三批神经毒素进行了分析。每批均从单独的产生神经毒素的细菌培养物中分离得到。两批接种物来自同一来源，另一批来自不同来源。两批神经毒素通过相同方法纯化，一批通过不同方法纯化。发现这些神经毒素制剂在纯度上相当，氨基酸组成相似。每个神经毒素分子（分子量155,000）的氨基酸残基数量的最佳估计值为：天冬氨酸218、苏氨酸80、丝氨酸105、谷氨酸128、脯氨酸47、甘氨酸69、丙氨酸47、缬氨酸72、半胱氨酸9、甲硫氨酸14、异亮氨酸128、亮氨酸104、酪氨酸86、苯丙氨酸60、赖氨酸90、组氨酸13、精氨酸51、色氨酸23。
