Spruce W E, McMillan R, Miller W, Fox R, Carson D, Schwartz D B, Hartman G A, Renshaw L W, Beutler E
Transplantation. 1983 Oct;36(4):369-72. doi: 10.1097/00007890-198310000-00004.
Four patients with acute leukemia received transplants from HLA-mismatched, related donors. Marrow cells that had been depleted of T lymphocytes using a monoclonal anti-T-lymphocyte antibody and rabbit complement were used. In vitro studies showed that 80-97% of the mature T lymphocytes were removed using this procedure. Infusion of the treated marrow was accomplished without complications, and engraftment occurred in each case. Graft-versus-host disease occurred in 3 of the 4 patients. These results show that additional manipulations of the marrow will be required to allow complete T lymphocyte removal from the marrow before the feasibility of this approach in HLA-mismatched patients can be determined.
4例急性白血病患者接受了来自HLA不匹配的相关供者的移植。使用单克隆抗T淋巴细胞抗体和兔补体对骨髓细胞进行T淋巴细胞清除。体外研究表明，采用该方法可清除80% - 97%的成熟T淋巴细胞。输注处理后的骨髓未出现并发症，且每例均发生植入。4例患者中有3例发生移植物抗宿主病。这些结果表明，在确定该方法对HLA不匹配患者的可行性之前，需要对骨髓进行额外操作，以实现从骨髓中完全清除T淋巴细胞。
