Grandmont A M, Chapdelaine P, Tremblay R R
Can J Biochem Cell Biol. 1983 Jul;61(7):764-9. doi: 10.1139/o83-096.
The use of a synthetic substrate (p-nitrophenyl-alpha-D-glucoside) to measure alpha-glucosidase activity has allowed us to demonstrate the presence of acid and neutral alpha-glucosidases in the reproductive organs of the male rat. Both enzymes increased in the epididymis, particularly in the caput segment, along with initiation of spermatogenesis at puberty; it then started decreasing after 12 weeks of life. Similar variations were not recorded in testis, prostate, and seminal vesicles. Castration led to a significant decrease of acid and neutral alpha-glucosidases in all accessory reproductive organs, but administration of testosterone proprionate (50 micrograms/day for 10 days) restored the enzyme activity to its original level. When estradiol-17 beta (5 mg) was administered simultaneously with testosterone (500 micrograms), the antagonistic effect of estradiol on testosterone was particularly evident on the levels of neutral alpha-glucosidases which reached the castration range, while the acid alpha-glucosidase remained unchanged in epididymis, prostate, and seminal vesicles. These results show that both acid and neutral alpha-glucosidases may be influenced by gonadal hormones in the male rat.
使用合成底物（对硝基苯基-α-D-葡萄糖苷）来测量α-葡萄糖苷酶活性，使我们能够证明雄性大鼠生殖器官中存在酸性和中性α-葡萄糖苷酶。随着青春期精子发生的开始，这两种酶在附睾中均增加，尤其是在附睾头段；然后在出生12周后开始下降。在睾丸、前列腺和精囊中未记录到类似的变化。去势导致所有附属生殖器官中的酸性和中性α-葡萄糖苷酶显著降低，但给予丙酸睾酮（50微克/天，持续10天）可使酶活性恢复到原始水平。当与睾酮（500微克）同时给予雌二醇-17β（5毫克）时，雌二醇对睾酮的拮抗作用在中性α-葡萄糖苷酶水平上尤为明显，其达到去势范围，而附睾、前列腺和精囊中的酸性α-葡萄糖苷酶保持不变。这些结果表明，酸性和中性α-葡萄糖苷酶在雄性大鼠中可能受性腺激素影响。
